Monoclonal anti-Human, TDP1 antibody (clone AT1F2)

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Overview

Product name Monoclonal anti-Human, TDP1 antibody (clone AT1F2)
Catalog no 4ab060137
Size 50ul
Background Tyrosyl-DNA phosphodiesterase (TDP1) is a DNA repair enzyme that catalyzes the hydrolysis of a phophodiester bond between a tyrosine residue and a DNA 3' phosphate. This protein is a member of the phospholipase D (PLD) superfamily and contains two PLD phosphodiesterase domains. Mutations in TDP1 are associated with the disease spinocerebellar ataxia with axonal neuropathy (SCAN1) by interfering with DNA transcription or by inducting apoptosis in postmitotic neurons.
Storage Liquid. In Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% Glycerol.
Predict MW
Synonyms tyrosyl-DNA phosphodiesterase 1, FLJ11090, SCAN1

Applications

Conjugate Unconjugated
Tested application ELISA, WB, Flow cytometry, ICC/IF
Clonality Monoclonal
Isotype IgG2b, k
Host mouse
Immunogen Recombinant human TDP1 (1-298aa) purified from E. coli
Reactivity Human

image of Monoclonal anti-Human, TDP1 antibody (clone AT1F2)

应用 Ab评论 说明
IHC-Fr 1/200.
CHIPseq Use at an assay dependent concentration.
Dot blot 1/10000.
IHC-P 1/100 - 1/400. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Electron 1/50. Customer feedback
ChIP Use 2µg for 106 cells
IP Use a concentration of 5 µg/ml.
WB 1/1000 - 1/5000. Detects a band of approximately 17 kDa (predicted molecular weight: 15 kDa).
ChIP/Chip Use at an assay dependent concentration.
IHC - Use at an assay dependent concentration. PubMed: 22219645
ICC 1/100 - 1/500.

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