SMCC活化的Percp, SMCC-Activated PerCP

¥8000.00

SMCC活化的Percp, SMCC-Activated PerCP

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Overview

Product name SMCC活化的Percp, SMCC-Activated PerCP
Catalog no APF004
Size 1mg
Description PerCP (Peridinin-chlorophyll-protein complex) is isolated from Dinophyceae sp. It has an extremely high extinction coefficient, a high quantum efficiency and a large Stokes shift. It is well excited with the Argon laser at 488 nm with its maximum emission peak at 677nm.The molecular weight of intact PerCP has been found to be about 35,000 daltons. PerCP has an extremely high absorbtivity, a high quantum efficiency, a large Stokes shift and a strong excitation peak at 482nm with its maximum emission peak at 677nm. The protein is commonly used for fluorescent immunolabeling, particularly in applications involving fluorescent-activated cell sorting (FACS). PerCP is useful fluorochromes for multicolor analysis with FITC, PE and other fluorochromes. Sulfo-SMCC are heterobifunctional crosslinkers that contain N-hydroxysuccinimide (NHS) ester and maleimide groups. NHS esters react with primary amines of PerCP at pH 7-9 to form amide bonds, while maleimides react with sulfhydryl groups at pH 6.5-7.5 to form stable thioether bonds. SMCC-activated PerCP is a stable protein which can be easily linked to antibodies and other sulfhydryl-containing proteins without altering its spectral characteristics. Excess non-reacted Sulfo-SMCC and by-products have been removed.

image of SMCC活化的Percp, SMCC-Activated PerCP

应用 Ab评论 说明
IHC-Fr 1/200.
CHIPseq Use at an assay dependent concentration.
Dot blot 1/10000.
IHC-P 1/100 - 1/400. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Electron 1/50. Customer feedback
ChIP Use 2µg for 106 cells
IP Use a concentration of 5 µg/ml.
WB 1/1000 - 1/5000. Detects a band of approximately 17 kDa (predicted molecular weight: 15 kDa).
ChIP/Chip Use at an assay dependent concentration.
IHC - Use at an assay dependent concentration. PubMed: 22219645
ICC 1/100 - 1/500.

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