SMCC活化的R-PE, SMCC-Activated R-PE


SMCC活化的R-PE, SMCC-Activated R-PE





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Product name SMCC活化的R-PE, SMCC-Activated R-PE
Catalog no APF001
Size 1mg
Description R-Phycoerythrin (R-PE) was originally isolated from red algae and has thress types of subunits: α (~ 20,000 daltons), β (~ 20,000 daltons) and γ (~ 30,000 daltons). The molecular weight of intact R-PE has been found to be about 240,000 daltons, and a subunit structure of (αβ)6γ has been determined. Its primary absorbance peak occurs at 565nm with secondary peaks at 495 and 545nm; the relative prominence of the secondary peaks varies significantly among R-PEs from different species. RPE has been used in a variety of immunological assays and as a fluorescent label for Fluorescence Activated Cell Sorting (FACS). Nonetheless, by virtue of its high absorption coefficient and almost perfect quantum efficiency, it is one of the brightest dyes available today. In addition, because of the high molar absorptivity of these proteins at visible wavelengths, they are convenient markers in such applications as gel electrophoresis, isoelectric focusing and gel exclusion chromatography. Sulfo-SMCC are heterobifunctional crosslinkers that contain N-hydroxysuccinimide (NHS) ester and maleimide groups. NHS esters react with primary amines of R-PE at pH 7-9 to form amide bonds, while maleimides react with sulfhydryl groups at pH 6.5-7.5 to form stable thioether bonds. SMCC-activated R-PE is a stable protein which can be easily linked to antibodies and other sulfhydryl-containing proteins without altering its spectral characteristics. Excess non-reacted Sulfo-SMCC and by-products have been removed.

image of SMCC活化的R-PE, SMCC-Activated R-PE

应用 Ab评论 说明
IHC-Fr 1/200.
CHIPseq Use at an assay dependent concentration.
Dot blot 1/10000.
IHC-P 1/100 - 1/400. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Electron 1/50. Customer feedback
ChIP Use 2µg for 106 cells
IP Use a concentration of 5 µg/ml.
WB 1/1000 - 1/5000. Detects a band of approximately 17 kDa (predicted molecular weight: 15 kDa).
ChIP/Chip Use at an assay dependent concentration.
IHC - Use at an assay dependent concentration. PubMed: 22219645
ICC 1/100 - 1/500.

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